Review



mouse recombinant dkk1  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    R&D Systems mouse recombinant dkk1
    Mouse Recombinant Dkk1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+dkk1/Recombinant+Mouse+Dkk-1+Protein/pm41673413-181-0-5
    Average 94 stars, based on 85 article reviews
    mouse recombinant dkk1 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Recombinant:

    Article Title: Icariin ameliorates glycolytic dysfunction in Alzheimer's disease models by activating the Wnt/β-catenin signaling pathway.
    Article Snippet: 1 Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, China 2 Department of Hospital Infection Management, People’s Hospital of WeiNing County, Bijie, China 3 Department of Clinical Medicine, Zunyi Medical University, China 4 Department of Gastroenterology, Digestive Disease Hospital, Affiliated Hospital of Zunyi Medical University, China

    Article Title: Coordinate control of basal epithelial cell fate and stem cell maintenance by core EMT transcription factor Zeb1
    Article Snippet: .. For basal MEC transplantation with Dkk1-coated beads, Affi-Gel blue beads (Bio-Rad Laboratories, 1537301) were washed three times with PBS and incubated with 1 mg/ml recombinant mouse Dkk1 (R&D Systems, 5897-DK-010) in 0.1% BSA for 1 hour before transplantation. ..

    Article Title: Inactivation of Sufu in cementoblasts accelerates external tooth root resorption.
    Article Snippet: Funding information National Research Foundation of Korea Abstract Cementum has been empirically regarded as an antiresorptive barrier against tooth roots.. However, little is known about the factors of homeostasis and resistant mechanisms of tooth roots against resorption.. Here, we investigated cementum factors and their interaction against resorption using transgenic mice exhibiting external cervical root resorption (ECRR).

    Article Title: Wnt/ β -catenin signaling regulates brain-derived neurotrophic factor release from spinal microglia to mediate HIV 1 gp120-induced neuropathic pain
    Article Snippet: HIV 1 gp120 (Bal) (HIV 1 /Clade B) (IT-001-002p) was purchased from Immune Technology Corp (USA). gp120 purified protein was reconstituted at 1 mg/mL in sterile filtered PBS (0.22-μm pore filters) only and aliquoted on ice (5 μL/tube) and stored at −80°C. .. Before usage, HIV 1 gp120 was immediately diluted to a final concentration of 20 ng/μL in PBS (100 ng/animal)., Recombinant human BDNF (Bachem: 4038290, 5 ng/animal), recombinant mouse Wnt3a (R&D: 1324-WN-010/CF, 100 ng/animal), recombinant mouse DKK1 (R&D: 5897-DK-010, 1 μg/animal), recombinant human TrkB-Fc (R&D: 688-TK-100, 250 ng/animal), or TAT-Pep5 (Sigma: 506181, 40 μg/animal) was first dissolved as a concentrated stock solution in 0.01 M PBS. .. IWR-1 (Sigma: I0161, 50 μM/animal) or minocycline (Sigma: M9511, 50 μg/animal) were dissolved in DMSO or in 0.9% sterile physiological saline., All the drugs were aliquoted in small volumes and stored at −80°C.

    Article Title: Dickkopf-1 Acts as a Profibrotic Mediator in Progressive Chronic Kidney Disease.
    Article Snippet: .. 2023, 24, 7679 15 of 18 experiments, mouse mesangial cells were cultured in medium supplemented with human serum, or were treated with anti-DKK1 antibody (#AF1096; R&D Systems, Minneapolis, MN, USA), normal IgG control (#AB-108-C; R&D Systems, Minneapolis, MN, USA), recombinant human DKK1 (5439-DK; R&D Systems, Minneapolis, MN, USA) or recombinant mouse DKK1 (5897-DK; R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Coordinate control of basal epithelial cell fate and stem cell maintenance by core EMT transcription factor Zeb1.
    Article Snippet: .. For basal MEC transplantation with Dkk1-coated beads, Affi-Gel blue beads (Bio-Rad Laboratories, 1537301) were washed three times with PBS and incubated with 1 mg/ml recombinant mouse Dkk1 (R&D Systems, 5897-DK-010) in 0.1% BSA for 1 hour before transplantation. ..

    Article Title: (Pro)renin receptor accelerates development of sarcopenia via activation of Wnt/YAP signaling axis
    Article Snippet: Intramuscular injection of DKK-1, verteporfin, and anti-(P)RR neutralizing antibody The reagents diluted in PBS were mixed with a thermo-reversible gelation polymer (Mebiol® Gel, Advanced BioMatrix, San Diego, CA, USA) and injected into the TA muscle of (P)RR-Tg mice or WT mice using a 29-gauge needle. .. The final concentrations of recombinant mouse DKK1 (R&D Systems), verteporfin (Chemscene, Monmouth Junction, NJ, USA), and anti-(P)RR neutralizing antibody (a kind gift from Dr. A. Nishiyama, Kagawa University, Japan) were adjusted to 500 ng/50 μL, 100 μg/50 μL, and 67 μg/50 μL, respectively. ..

    Article Title: Dickkopf-1 Acts as a Profibrotic Mediator in Progressive Chronic Kidney Disease
    Article Snippet: Mouse mesangial cell line SV40 MES 13 (ATCC; CRL-1927) was routinely cultured in DME/F-12 (3:1 mixture) medium supplemented with 5% fetal bovine serum. .. In some experiments, mouse mesangial cells were cultured in medium supplemented with human serum, or were treated with anti-DKK1 antibody (#AF1096; R&D Systems, Minneapolis, MN, USA), normal IgG control (#AB-108-C; R&D Systems, Minneapolis, MN, USA), recombinant human DKK1 (5439-DK; R&D Systems, Minneapolis, MN, USA) or recombinant mouse DKK1 (5897-DK; R&D Systems, Minneapolis, MN, USA). ..

    Transplantation Assay:

    Article Title: Coordinate control of basal epithelial cell fate and stem cell maintenance by core EMT transcription factor Zeb1
    Article Snippet: .. For basal MEC transplantation with Dkk1-coated beads, Affi-Gel blue beads (Bio-Rad Laboratories, 1537301) were washed three times with PBS and incubated with 1 mg/ml recombinant mouse Dkk1 (R&D Systems, 5897-DK-010) in 0.1% BSA for 1 hour before transplantation. ..

    Article Title: Coordinate control of basal epithelial cell fate and stem cell maintenance by core EMT transcription factor Zeb1.
    Article Snippet: .. For basal MEC transplantation with Dkk1-coated beads, Affi-Gel blue beads (Bio-Rad Laboratories, 1537301) were washed three times with PBS and incubated with 1 mg/ml recombinant mouse Dkk1 (R&D Systems, 5897-DK-010) in 0.1% BSA for 1 hour before transplantation. ..

    Incubation:

    Article Title: Coordinate control of basal epithelial cell fate and stem cell maintenance by core EMT transcription factor Zeb1
    Article Snippet: .. For basal MEC transplantation with Dkk1-coated beads, Affi-Gel blue beads (Bio-Rad Laboratories, 1537301) were washed three times with PBS and incubated with 1 mg/ml recombinant mouse Dkk1 (R&D Systems, 5897-DK-010) in 0.1% BSA for 1 hour before transplantation. ..

    Article Title: Coordinate control of basal epithelial cell fate and stem cell maintenance by core EMT transcription factor Zeb1.
    Article Snippet: .. For basal MEC transplantation with Dkk1-coated beads, Affi-Gel blue beads (Bio-Rad Laboratories, 1537301) were washed three times with PBS and incubated with 1 mg/ml recombinant mouse Dkk1 (R&D Systems, 5897-DK-010) in 0.1% BSA for 1 hour before transplantation. ..

    Concentration Assay:

    Article Title: Wnt/ β -catenin signaling regulates brain-derived neurotrophic factor release from spinal microglia to mediate HIV 1 gp120-induced neuropathic pain
    Article Snippet: HIV 1 gp120 (Bal) (HIV 1 /Clade B) (IT-001-002p) was purchased from Immune Technology Corp (USA). gp120 purified protein was reconstituted at 1 mg/mL in sterile filtered PBS (0.22-μm pore filters) only and aliquoted on ice (5 μL/tube) and stored at −80°C. .. Before usage, HIV 1 gp120 was immediately diluted to a final concentration of 20 ng/μL in PBS (100 ng/animal)., Recombinant human BDNF (Bachem: 4038290, 5 ng/animal), recombinant mouse Wnt3a (R&D: 1324-WN-010/CF, 100 ng/animal), recombinant mouse DKK1 (R&D: 5897-DK-010, 1 μg/animal), recombinant human TrkB-Fc (R&D: 688-TK-100, 250 ng/animal), or TAT-Pep5 (Sigma: 506181, 40 μg/animal) was first dissolved as a concentrated stock solution in 0.01 M PBS. .. IWR-1 (Sigma: I0161, 50 μM/animal) or minocycline (Sigma: M9511, 50 μg/animal) were dissolved in DMSO or in 0.9% sterile physiological saline., All the drugs were aliquoted in small volumes and stored at −80°C.

    Cell Culture:

    Article Title: Dickkopf-1 Acts as a Profibrotic Mediator in Progressive Chronic Kidney Disease.
    Article Snippet: .. 2023, 24, 7679 15 of 18 experiments, mouse mesangial cells were cultured in medium supplemented with human serum, or were treated with anti-DKK1 antibody (#AF1096; R&D Systems, Minneapolis, MN, USA), normal IgG control (#AB-108-C; R&D Systems, Minneapolis, MN, USA), recombinant human DKK1 (5439-DK; R&D Systems, Minneapolis, MN, USA) or recombinant mouse DKK1 (5897-DK; R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Dickkopf-1 Acts as a Profibrotic Mediator in Progressive Chronic Kidney Disease
    Article Snippet: Mouse mesangial cell line SV40 MES 13 (ATCC; CRL-1927) was routinely cultured in DME/F-12 (3:1 mixture) medium supplemented with 5% fetal bovine serum. .. In some experiments, mouse mesangial cells were cultured in medium supplemented with human serum, or were treated with anti-DKK1 antibody (#AF1096; R&D Systems, Minneapolis, MN, USA), normal IgG control (#AB-108-C; R&D Systems, Minneapolis, MN, USA), recombinant human DKK1 (5439-DK; R&D Systems, Minneapolis, MN, USA) or recombinant mouse DKK1 (5897-DK; R&D Systems, Minneapolis, MN, USA). ..

    Control:

    Article Title: Dickkopf-1 Acts as a Profibrotic Mediator in Progressive Chronic Kidney Disease.
    Article Snippet: .. 2023, 24, 7679 15 of 18 experiments, mouse mesangial cells were cultured in medium supplemented with human serum, or were treated with anti-DKK1 antibody (#AF1096; R&D Systems, Minneapolis, MN, USA), normal IgG control (#AB-108-C; R&D Systems, Minneapolis, MN, USA), recombinant human DKK1 (5439-DK; R&D Systems, Minneapolis, MN, USA) or recombinant mouse DKK1 (5897-DK; R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Dickkopf-1 Acts as a Profibrotic Mediator in Progressive Chronic Kidney Disease
    Article Snippet: Mouse mesangial cell line SV40 MES 13 (ATCC; CRL-1927) was routinely cultured in DME/F-12 (3:1 mixture) medium supplemented with 5% fetal bovine serum. .. In some experiments, mouse mesangial cells were cultured in medium supplemented with human serum, or were treated with anti-DKK1 antibody (#AF1096; R&D Systems, Minneapolis, MN, USA), normal IgG control (#AB-108-C; R&D Systems, Minneapolis, MN, USA), recombinant human DKK1 (5439-DK; R&D Systems, Minneapolis, MN, USA) or recombinant mouse DKK1 (5897-DK; R&D Systems, Minneapolis, MN, USA). ..



    Similar Products

    94
    R&D Systems mouse recombinant dkk1
    Mouse Recombinant Dkk1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+dkk1/Recombinant+Mouse+Dkk-1+Protein/pm41673413-181-0-5
    Average 94 stars, based on 1 article reviews
    mouse recombinant dkk1 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    93
    R&D Systems dkk1 protein levels
    Dkk1 Protein Levels, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+dkk1/Recombinant+Mouse+Dkk-1+Protein%2C+CF/pm40057150-131-0-22
    Average 93 stars, based on 1 article reviews
    dkk1 protein levels - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    94
    R&D Systems dkk1 protein
    <t>DKK1</t> bead implantation interrupts fin ray outgrowth in the little skate. Compare the control bead ( A ) vs. DKK1-soaked beads ( B – F ). Note the reduced fin ray outgrowth in 100% embryos implanted with a DKK1-soaked bead ( n = 21), which is not observed with the control bead. The truncated fin rays observed in association with DKK1 bead implantations ( B – F ) resemble the shorter fin rays observed in the “notch” mirroring the early stages of pectoral fin domain splitting in the myliobatid phenotype (Fig. ). See text for explanation of why the phenotype could not be driven to completion due to limited activity of the ectopic DKK1 protein. Pictures show dorsal views of the anterior pectoral fins of six different little skate embryos that have been incubated for 4 weeks after bead implantation. Scale bar represents 2.5 mm
    Dkk1 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+dkk1/Recombinant+Mouse+Dkk-1+Protein/pmc11681717-66-18-22
    Average 94 stars, based on 1 article reviews
    dkk1 protein - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    R&D Systems recombinant mouse dkk1
    <t>DKK1</t> bead implantation interrupts fin ray outgrowth in the little skate. Compare the control bead ( A ) vs. DKK1-soaked beads ( B – F ). Note the reduced fin ray outgrowth in 100% embryos implanted with a DKK1-soaked bead ( n = 21), which is not observed with the control bead. The truncated fin rays observed in association with DKK1 bead implantations ( B – F ) resemble the shorter fin rays observed in the “notch” mirroring the early stages of pectoral fin domain splitting in the myliobatid phenotype (Fig. ). See text for explanation of why the phenotype could not be driven to completion due to limited activity of the ectopic DKK1 protein. Pictures show dorsal views of the anterior pectoral fins of six different little skate embryos that have been incubated for 4 weeks after bead implantation. Scale bar represents 2.5 mm
    Recombinant Mouse Dkk1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+dkk1/Recombinant+Mouse+Dkk-1+Protein/pm38400523-261-8-12
    Average 94 stars, based on 1 article reviews
    recombinant mouse dkk1 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    90
    ABclonal Biotechnology recombinant mouse dkk1
    <t>DKK1</t> bead implantation interrupts fin ray outgrowth in the little skate. Compare the control bead ( A ) vs. DKK1-soaked beads ( B – F ). Note the reduced fin ray outgrowth in 100% embryos implanted with a DKK1-soaked bead ( n = 21), which is not observed with the control bead. The truncated fin rays observed in association with DKK1 bead implantations ( B – F ) resemble the shorter fin rays observed in the “notch” mirroring the early stages of pectoral fin domain splitting in the myliobatid phenotype (Fig. ). See text for explanation of why the phenotype could not be driven to completion due to limited activity of the ectopic DKK1 protein. Pictures show dorsal views of the anterior pectoral fins of six different little skate embryos that have been incubated for 4 weeks after bead implantation. Scale bar represents 2.5 mm
    Recombinant Mouse Dkk1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+dkk1/recombinant+mouse+dkk1/pm37946278-102-111-116
    Average 90 stars, based on 1 article reviews
    recombinant mouse dkk1 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    DKK1 bead implantation interrupts fin ray outgrowth in the little skate. Compare the control bead ( A ) vs. DKK1-soaked beads ( B – F ). Note the reduced fin ray outgrowth in 100% embryos implanted with a DKK1-soaked bead ( n = 21), which is not observed with the control bead. The truncated fin rays observed in association with DKK1 bead implantations ( B – F ) resemble the shorter fin rays observed in the “notch” mirroring the early stages of pectoral fin domain splitting in the myliobatid phenotype (Fig. ). See text for explanation of why the phenotype could not be driven to completion due to limited activity of the ectopic DKK1 protein. Pictures show dorsal views of the anterior pectoral fins of six different little skate embryos that have been incubated for 4 weeks after bead implantation. Scale bar represents 2.5 mm

    Journal: EvoDevo

    Article Title: The evolution of cephalic fins in manta rays and their relatives: functional evidence for initiation of domain splitting and modulation of the Wnt signaling pathway in the pectoral fin AER of the little skate

    doi: 10.1186/s13227-024-00233-3

    Figure Lengend Snippet: DKK1 bead implantation interrupts fin ray outgrowth in the little skate. Compare the control bead ( A ) vs. DKK1-soaked beads ( B – F ). Note the reduced fin ray outgrowth in 100% embryos implanted with a DKK1-soaked bead ( n = 21), which is not observed with the control bead. The truncated fin rays observed in association with DKK1 bead implantations ( B – F ) resemble the shorter fin rays observed in the “notch” mirroring the early stages of pectoral fin domain splitting in the myliobatid phenotype (Fig. ). See text for explanation of why the phenotype could not be driven to completion due to limited activity of the ectopic DKK1 protein. Pictures show dorsal views of the anterior pectoral fins of six different little skate embryos that have been incubated for 4 weeks after bead implantation. Scale bar represents 2.5 mm

    Article Snippet: O’Shaughnessy (personal communication) with the following modifications: Affigel-Blue (Bio-Rad, 1537302) beads were soaked overnight at 4 °C in DKK1 protein (mouse origin; R&D Systems, 5897-DK-010) reconstituted at 100 μg/mL in PBS containing 0.1% bovine serum albumin (BSA) or, as a control, in PBS containing 0.1% BSA.

    Techniques: Control, Activity Assay, Incubation

    Ectopic DKK1 interrupts AER maintenance/ Axin2 expression in the little skate pectoral fin for up to 72 h. Compare left column with interrupted Axin2 expression near the Dkk1 soaked bead with the right column that shows stage-specific native expression with implantation of a control bead. Axin2 expression is depicted in the anterior pectoral fins of little skate embryos at stages 30 ( A , B ; G , H ) and 31 ( C – F ; I , J ) of development after implantation of agarose beads soaked in DKK1 protein in the left fin (left column) and PBS in the right fin (right column) with the following incubation times: 6 h ( A , B ), 12 h ( C , D ), 24 h ( E , F ), 36 h ( G , H ) and 72 h ( I , J ). Comparisons between the DKK1 (left pectoral fin) and PBS control (right pectoral fin) beads illustrate treatment effects from implantations in the same individual. In some individuals ( C , D and G , H ), beads came out during the ISH process. Former location of beads is visible and denoted by blue dashed circles. Solid purple circles denote regions with Axin2 expression and dashed purple circles denote regions where Axin2 expression is expected but has been interrupted. For all incubation times, PBS beads had no effect on Axin2 expression. After 6 h, interruption of AER-associated Axin2 expression in the DKK1 treatment is clearly visible near the bead ( A , B ). After 12, 24, and 36 h incubation times, Axin2 expression in the anterior pectoral fins was completely interrupted. After 72 h of incubation ( I , J ), the effect of DKK1 inhibition on Axin2 is diminished but still visible near the bead, suggesting that the temporal scale of DKK1 protein activity is approximately 1–3 days. Axin2 expression in the posterior pectoral fins ( L , M ) was not affected in any individual regardless of treatment or incubation time, suggesting a spatial scale of approximately 1 cm from the implanted bead. Posterior pectoral fins at 24 hpi are depicted as this is when DKK1 reaches peak penetrance. Sense probe (see B ) showed no staining. Since embryos with different incubation times were at different developmental stages, the expected expression domain of Axin2 varies accordingly. Scale bar represents 1 mm

    Journal: EvoDevo

    Article Title: The evolution of cephalic fins in manta rays and their relatives: functional evidence for initiation of domain splitting and modulation of the Wnt signaling pathway in the pectoral fin AER of the little skate

    doi: 10.1186/s13227-024-00233-3

    Figure Lengend Snippet: Ectopic DKK1 interrupts AER maintenance/ Axin2 expression in the little skate pectoral fin for up to 72 h. Compare left column with interrupted Axin2 expression near the Dkk1 soaked bead with the right column that shows stage-specific native expression with implantation of a control bead. Axin2 expression is depicted in the anterior pectoral fins of little skate embryos at stages 30 ( A , B ; G , H ) and 31 ( C – F ; I , J ) of development after implantation of agarose beads soaked in DKK1 protein in the left fin (left column) and PBS in the right fin (right column) with the following incubation times: 6 h ( A , B ), 12 h ( C , D ), 24 h ( E , F ), 36 h ( G , H ) and 72 h ( I , J ). Comparisons between the DKK1 (left pectoral fin) and PBS control (right pectoral fin) beads illustrate treatment effects from implantations in the same individual. In some individuals ( C , D and G , H ), beads came out during the ISH process. Former location of beads is visible and denoted by blue dashed circles. Solid purple circles denote regions with Axin2 expression and dashed purple circles denote regions where Axin2 expression is expected but has been interrupted. For all incubation times, PBS beads had no effect on Axin2 expression. After 6 h, interruption of AER-associated Axin2 expression in the DKK1 treatment is clearly visible near the bead ( A , B ). After 12, 24, and 36 h incubation times, Axin2 expression in the anterior pectoral fins was completely interrupted. After 72 h of incubation ( I , J ), the effect of DKK1 inhibition on Axin2 is diminished but still visible near the bead, suggesting that the temporal scale of DKK1 protein activity is approximately 1–3 days. Axin2 expression in the posterior pectoral fins ( L , M ) was not affected in any individual regardless of treatment or incubation time, suggesting a spatial scale of approximately 1 cm from the implanted bead. Posterior pectoral fins at 24 hpi are depicted as this is when DKK1 reaches peak penetrance. Sense probe (see B ) showed no staining. Since embryos with different incubation times were at different developmental stages, the expected expression domain of Axin2 varies accordingly. Scale bar represents 1 mm

    Article Snippet: O’Shaughnessy (personal communication) with the following modifications: Affigel-Blue (Bio-Rad, 1537302) beads were soaked overnight at 4 °C in DKK1 protein (mouse origin; R&D Systems, 5897-DK-010) reconstituted at 100 μg/mL in PBS containing 0.1% bovine serum albumin (BSA) or, as a control, in PBS containing 0.1% BSA.

    Techniques: Expressing, Control, Incubation, Inhibition, Activity Assay, Staining

    Dkk1 expression modifies AER outgrowth during pectoral fin development. Here, we demonstrate Dkk1 expression in cownose ray cephalic fins at stage 3 (stage 31 in skates), the only stage available for this experiment, which is after development of the “notch”. A No staining with the Dkk1 control sense probe. B , C Expression in the distal ridge of the AER indicated by purple staining with the Dkk1 antisense experimental probe on the left side ( B ), and right side ( C ). Purple arrows denote regions where Dkk1 expression occurs. While Dkk1 was expressed in the “notch” region at an earlier stage (based on ), it is still expressed in the cephalic fin AER where fin ray length is somewhat reduced. Scale bar represents 2.5 mm

    Journal: EvoDevo

    Article Title: The evolution of cephalic fins in manta rays and their relatives: functional evidence for initiation of domain splitting and modulation of the Wnt signaling pathway in the pectoral fin AER of the little skate

    doi: 10.1186/s13227-024-00233-3

    Figure Lengend Snippet: Dkk1 expression modifies AER outgrowth during pectoral fin development. Here, we demonstrate Dkk1 expression in cownose ray cephalic fins at stage 3 (stage 31 in skates), the only stage available for this experiment, which is after development of the “notch”. A No staining with the Dkk1 control sense probe. B , C Expression in the distal ridge of the AER indicated by purple staining with the Dkk1 antisense experimental probe on the left side ( B ), and right side ( C ). Purple arrows denote regions where Dkk1 expression occurs. While Dkk1 was expressed in the “notch” region at an earlier stage (based on ), it is still expressed in the cephalic fin AER where fin ray length is somewhat reduced. Scale bar represents 2.5 mm

    Article Snippet: O’Shaughnessy (personal communication) with the following modifications: Affigel-Blue (Bio-Rad, 1537302) beads were soaked overnight at 4 °C in DKK1 protein (mouse origin; R&D Systems, 5897-DK-010) reconstituted at 100 μg/mL in PBS containing 0.1% bovine serum albumin (BSA) or, as a control, in PBS containing 0.1% BSA.

    Techniques: Expressing, Staining, Control